| mAbScreen: New drug-screening platform for therapeutic monoclonal antibodies |
Uses AP-TAG technology for clone selection
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 | | mAbScreen |
GenHunter has developed a new therapeutic monoclonal antibody screening platform (dubbed mAbScreeen = Monoclonal Antibody Screening).
Current methods for the detection and quantification of therapeutic antibodies and Fc fusion proteins rely on the traditional sandwich ELISA method that has to be customized for each individual biologic drug. For example, there are currently multiple TNF-a blockers on the market, including Remicade, Humira, and Enbrel, each of which has its own ELISA method of detection, employing different antibodies.
Using our patented AP-TAG (alkaline phosphatase tag) technology (US patents 5,554,499 and 5,801,000), we have developed the new mAbScreen technology platform, which allows for more accurate, rapid, economical, generic, and ligand-specific detection and quanti...more
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| Trimer-Tag: New technology for Trimerization of Secreted Proteins |
Covered by 3 US patents
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 | | Trimeric receptor bound to ligand |
GenHunter Corporation has been granted 3 U.S. patents (7,268,116; 7,666,837; and 7,691,815) covering the design and expression of any secreted therapeutic protein in disulfide bond-linked homo-trimeric forms.
The new technology dubbed "Trimer-Tag" takes advantage of the C-prodomains of collagen proteins, which are capable of efficient self-trimerization. Collagens are the most abundant and naturally occurring proteins secreted by mammals, including humans.
Trimer-Tag is the first drug design platform that allows any secreted protein (e.g. cytokines, soluble receptors, Fab of mAbs) to be made as covalently linked homo-trimers with greatly increased avidity to their disease-causing targets such as the TNF family of cytokines (TNFRII, TRAIL, etc), HIV gp120, DR4, DR...more
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| Who really invented the Western blot? |
Learn the actual story behind the western blot technique
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Was it Neal Burnette (at Fred Hutchinson)?
Was it Harry Towbin's group (in Switzerland)?
Was it George Stark's group (at Stanford)?
1979 certainly was a busy year in the development of western blotting (also called "Protein blotting" or "Protein immunoblot"). They were all working hard and trying to publish.
But who deserves the real credit?
Most researchers know western blotting evolved from Southern blotting (Ref 1), invented by Edwin Southern at University of Edinburgh in 1975, then northern blotting (Ref 2), invented by George Stark's Stanford group in 1977.
All 3 groups of researchers in Seattle, Stanford, and Basel seem to have been working independently from 1977 to 1979 on a better method to detect proteins using antibodies. They all attempted to publish in 1979 and certainly all deserve some credit for the development of the western blot. But who should we cite as the real inventor? This question is not as easy to answer, but it is a very interesting story....more
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